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鏈霉素(Strep)ELISA檢測(cè)試劑盒（檢雞肉組織/牛奶/蜂蜜，靈敏度0.1ppb,兩步法）

鏈霉素(Strep)ELISA快速檢測(cè)試劑盒

一、原理

本試劑盒采用間接競(jìng)爭(zhēng)ELISA方法，在微孔條上預(yù)包被偶聯(lián)抗原，樣本中的殘留物鏈霉素將和微孔條上預(yù)包被的偶聯(lián)抗原競(jìng)爭(zhēng)抗鏈霉素抗體，加入酶標(biāo)記物后，用TMB底物顯色，樣本吸光值與其所含殘留物鏈霉素的含量成負(fù)相關(guān)，與標(biāo)準(zhǔn)曲線比較即可得出相應(yīng)殘留物鏈霉素的含量。

二、試劑盒特性

u  試劑盒靈敏度：   0.1ppb

u  孵育溫度 ：      37℃

u  孵育時(shí)間 ：      30min～30min～15min

批內(nèi)、批間差：      <10%

u  樣本檢測(cè)下限

雞 肉        ······································  1 ppb

雞肝、牛奶   ······································  4 ppb

蜂 蜜/蜂王漿 ······································  2 ppb

u  交叉反應(yīng)率

鏈霉素  ·············································  100%

慶大霉素  ············································ ﹤0.1%

雙氫鏈霉素  ···········································  108%

卡那霉素  ············································ ﹤0.1%

u  樣本回收率

牛    奶  ············································  85±22%

雞肉組織  ············································  80±22%

蜂蜜/蜂王漿   ··········································  75±22%

三、試劑盒組成

四、樣品前處理步驟及檢測(cè)分析步驟

  詳見(jiàn)產(chǎn)品說(shuō)明書。

五、儲(chǔ)藏條件和保質(zhì)期

1、 儲(chǔ)藏條件：試劑盒于2～8℃保存，不要冷凍。

2、 保 質(zhì) 期：該產(chǎn)品有效期為1年，生產(chǎn)日期見(jiàn)包裝盒。

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